National Heart, Lung, and Blood Institute program in
Acute Lung Injury
Specialized Centers of Clinically Oriented Research (ALI SCCOR)

1987-1990           Intern, Assistant Resident, Senior Resident, Massachusetts General Hospital, Boston, MA
1990-1991           Chief Resident in Medicine,University of Washington Medical Center, Seattle, WA
1990-1994           Acting Instructor of Medicine (90-92), Sr Fellow, Infectious Diseases (91-94), Univ of Washington
1994-1996           Acting Instructor of Medicine, Infectious Diseases, University of Washington
1996-2000           Assistant Professor of Medicine, Infectious Diseases, University of Washington
2000-present        Associate Professor of Medicine, Infectious Diseases, University of Washington
2001-present        Adjunct Associate Professor of  Pathology, University of Washington

Honors:  National Merit Scholar (1976); Magna Cum Laude (1980); Alpha Omega Alpha (1986); Internal Medicine Prize, Medical Thesis Honors, Harold Lamport Biomedical Research Prize (1987); Fellow, American College of Physicians (1998); Fialkow Scholar Award (1998); Fellow, Infectious Diseases Society of America (2001); Outstanding Investigator Award, Western Society for Clinical Investigation (2004)

Study Sections

2000-present                        Grant Proposal Reviewer – The Wellcome Trust
2001-present                        Ad-hoc Reviewer, VA Merit Awards – Grant Applications
2001-present                        Fellowship & Young Investigator Grants Award Committee – IDSA
2003-present                        Grant Proposal Reviewer – Medical Research Council of Ireland
2003                                   NIH/NIAID Special Emphasis Panel/Initial Review Group 2004/01 ZAI1 RB-M (J1)
2003                                   NIH/NHLBI Cardiovascular Sciences Special Emphasis Panel ZRG1 PHRA 03
2004-present                        Grant Proposal Reviewer – Israel Science Foundation
2004-present                         NIH/NIAID Immunity and Host Defense Study Section ZRG1 IHD (01) Q

Selected peer-reviewed publications (in chronological order; selected from 138 publications)

1.            Imanishi T, Hano T, Nishio I,  Liles WC, Schwartz SM, Han DK. Transition of apoptotic resistant vascular smooth muscle cells to apoptotic sensitive state is correlated with down-regulation of c-FLIP.  J Vasc Res 2000; 37:523-531.

2.            Schaub FJ, Han DKM, Liles WC, Adams LD, Coats SA, Ramachandran RK, Seifert RA, Schwartz SM, Bowen-Pope DF. Fas/FADD-mediated activation of a specific program of inflammatory gene expression in vascular smooth muscle cells. Nature Med 2000; 6:790-796.

3.            Aprikyan AAG, Liles WC, Rodger E, Jonas M, Chi  EY, Dale DC. Imparied survival of bone marrow hematopoietic progenitor cells in cyclic neutropenia. Blood 2001; 97:147-153.

4.            Matute-Bello G, Winn RK, Chi EY, Martin TR, Liles WC.  Activation of Fas (CD95) induces acute lung inflammation in mice. Am J Pathol 2001; 158:153-161.

5.            Hubel K, Dale DC, Engert A, Liles WC. Current status of granulocyte (neutrophil) transfusion therapy for infectious diseases. J Infect Dis 2001; 183:321-328.

6.            Liles WC. Immunomodulatory approaches to augment phagocyte-mediated host defense. Sem Respir Infect 2001; 16:11-17.

7.            Matute-Bello G, Liles WC, Nakamura M, Frevert CW, Kiener PA, Ballman K, Vathanprida C, Martin TR. Recombinant human Fas ligand induces lung injury in rabbits. Am J Physiol Lung Cell Mol Physiol 2001; 281:L328-335.

8.            Matute-Bello G, Frevert CW, Liles WC, Nakamura M, Ruzinski JT, Ballman K, Wong V, Vathanprida C, Martin TR. The Fas/FasL system mediates epithelial injury, but not pulmonary host defenses, in response to experimental bacterial pneumonia in mice. Infect Immun 2001; 69:5768-5776.

9.            Imanishi T, Han DKM, Hofstra L,  Hano T, Nishio I, Liles WC, Kiener PA, Gown AM, Schwartz SM. Apoptosis of vascular smooth muscle cells is induced by Fas ligand derived from monocytes/macrophages. Atherosclerosis 2002; 161:143-151.

10.        Hubel K, Dale DC, Liles WC. Therapeutic use of cytokines to modulate phagocyte function for the treatment of infectious diseases – Current status of G-CSF, GM-CSF, M-CSF, and IFNg. J Infect Dis 2002; 185:1490-1501.

11.        Kaushansky K, Fox N, Lin NC, Liles WC. Lineage-specific growth factors can compensate for stem and progenitor cell deficiencies at the post-progenitor cell level: An analysis of doubly TPO- and G-CSF receptor-deficient mice. Blood 2002; 99:3373-3378.

12.        Marr KA, Balajee SA, Hawn TR, Ozinsky  A, Pham U, Aderem A, Liles WC. Effects of MyD88 on macrophage-mediated  responses to opportunistic fungal pathogens; Infect Immun 2003; 71:5280-5286.

13.        Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC. Fas (CD95) induces pro-inflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol 2003; 170:6209-6216.

14.        Aprikyan AAG, Kutyavin T, Stein S, Rodger E, Liles WC, Boxer LA, Dale DC. Cellular and molecular abnormalities in severe congenital neutropenia predisposing to leukemia. Exp Hematol 2003; 31:372-381.

15.      Nhan TQ, Liles WC, Chait A, Fallon JT, Schwartz SM. The p17 cleaved form of caspase-3 is present within viable macrophages in vitro and in atherosclerotic plaque. Arterioscler Thromb Vasc Res 2003; 23:1776-1782.

16.      Liles WC, Broxmeyer HE, Rodger E, Hubel K, Cooper S, Hangoc G, Bridger GJ, Henson GW, Calandra G, Dale DC. Leukocytosis and mobilization of pluripotent hematopoietic progenitor cells in healthy volunteers induced by single dose administration of AMD-3100, a CXCR4 antagonist. Blood 2003; 102:2728-2730.

17.      Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF. Fas/FADD regulates MCP-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of IL-1a. Circ Res 2003; 93:515-522.

18.      Matute-Bello G, Winn RK, Martin TR, Liles WC. Sustained LPS-induced lung inflammation in mice is attenuated by functional deficiency of the Fas/Fas ligand system. Clin Diag Lab Immunol 2004; 11:358-361.

19.      Nakamura M, Matute-Bello G, Liles WC, Kajikawa O, Lin S-M, Frevert CW, Martin TR. Differential response of human lung epithelial cells to Fas-induced apoptosis. Am J Pathol 2004; 164:1949-1958.

20.      Matute-Bello G, Lee JS, Frevert CW, Liles WC, Sutlief S, Ballman K, Selk A, Wong V, Martin TR. Optimal timing to repopulation of resident alveolar macrophages with donor cells following total body irradiation and bone marrow transplantation in mice. J Immunol Methods 2004; 292:25-34.

21.      Matute-Bello G, Liles WC, Frevert CW, Dhanireddy S, Nakamura M, Ballman K, Wong V, Song HY, Witcher DR, Jakubowski JA, Martin TR. Blockade of the Fas/FasL system by improves pneumococcal clearance from lung without preventing bacteremic dissemination to the spleen. J Infect Dis 2005; 191:596-606.

22.      Nhan TQ, Liles WC, Schwartz SM. Death and survival of the plaque macrophage. Arterioscler Thromb Vasc Biol 2005; in press.

23.      Liles WC, Rodger E, Broxmeyer HE, Srour EF, Dehner C, Badel K, Calandra G, Christensen J, Wood B, Price TH, Dale DC. Augmented mobilization and collection of CD34+ hematopoietic cells from normal volunteers stimulated with G-CSF by single-dose administration of AMD3100, a CXCR4 antagonist. Transfusion 2005;295-300.

24.      Broxmeyer HE, Clapp DW, Orscell CM, Hangoc G, Cooper S, Plett PA, Liles WC, Li X, Graham-Evans B, Calandrta G, Bridger G, Dale DC, Srour EF. Potent and rapid mobilization and synergistic augmentation of G-CSF-induced mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med 2005; 201:1307-1318.

25.      Yaraei K, Zhu X, Liles WC, Campbell LA, Kuo C-C, Rosenfeld ME. Chlamydia pneumoniae augments the death of oxidized LDL treated mouse macrophages by a Toll-like receptor 2-dependent, caspase-independent pathway. Infect Immun 2005; 73:4315-4322..

26.      Yaraei K, Zhu X, Liles WC, Campbell LA, Kuo C-C, Rosenfeld ME. Effect of Chlamydia pneumoniae on cellular ATP content in mouse macrophages: Role of Toll-like receptor 2. Infect Immun 2005; 73:4323-4326.

27.      Hubel K, Rodger E, Gaviria JM, Price TH, Dale DC, Liles WC. Effective storage of granulocytes collected by centrifugation leukapheresis from donors stimulated with granulocyte colony-stimulating factor (G-CSF). Transfusion 2005; in press.

28.      Altemeier WA, Matute-Bello G, Gharib SA, Glenny RW, Martin TR, Liles WC. Modulation of LPS-induced gene transcription and promotion of lung injury by mechanical ventilation. J Immunol 2005; in press.

29.      Matute-Bello G, Lee JS, Liles WC, Frevert CW, Sutlief S, Martin TR. Fas-mediated acute lung injury requires membrane Fas expression on non-myeloid cells of the lungs. J Immunol 2005; in press.

30.      Hawn TR, Ozinsky A, Rodriguez S, Clark A, Pham U, Hill HR, Ochs H, Aderem A, Liles WC. Hyper-IgE syndrome is not associated with genetic or functional defects in several candidate Toll-like receptor pathway genes. Hum Immunol 2005; in press.

31.      Kassim SY, Fu X, Liles WC, Shapiro SD, Parks WC, Heinecke JW. NADPH oxidase prevents emphysema by restraining the metalloproteinase activity of macrophages: Reactive oxygen species restrain the metalloproteinase activity of macrophages. J Biol Chem 2005; in press.